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GrainGenes Marker Report: Xgwm361

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Locus	Xgwm361
Type	Microsatellite
Chromosome	6B
Chromosome Arm	6BS
Map	Tt-LxG-6B 89 Wheat, UC1110 x PI610750 6B 66.1 Wheat-Composite2004-6B 106
	[ Show Nearby Loci ]
Map Data	T. turgidum, Langdon x G18-16 Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMS361
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm361-6B gwm361 gwm361-6B
Probe for Locus	WMS361
Locus	Xgwm361 Xgwm361-6B gwm361 gwm361-6B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)20imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTAACTTGTTGCCAAAGGGG 3' 5' ACAAAGTGGCAAAAGGAGACA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS361
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring