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GrainGenes Marker Report: Xgwm368

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Locus	Xgwm368
Type	Microsatellite
Chromosome	4B
Map	Wheat-Composite2004-4B 36
	[ Show Nearby Loci ]
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004
Probe	WMS368
Possible Orthologs	Xgwm368-4B gwm368 gwm368-4B
Probe for Locus	WMS368
Locus	Xgwm368-4B Xgwm368 gwm368 gwm368-4B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (AT)25 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CCATTTCACCTAATGCCTGC 3' 5' AATAAAACCATGAGCTCACTTGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS368
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring