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GrainGenes Marker Report: Xgwm383.2

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Locus
Xgwm383.2
Type
Microsatellite
Map
Ta-NxW-3D116.3
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMS383
Possible Orthologs
Xgwm383-3B
[ Show all 8 ]



Probe for Locus
WMS383
Locus
Xgwm383-3D
[ Show all 9 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)27
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' ACGCCAGTTGATCCGTAAAC 3'
5' GACATCAATAACCGTGGATGG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS383
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring