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GrainGenes Marker Report: Xgwm397

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Locus	Xgwm397
Type	Microsatellite
Map	Ta-NxW-4A 66.7 Wheat, UC1110 x PI610750 4A 19.8
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMS397
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm397-4A Xgwm397a gwm397
Probe for Locus	WMS397
Locus	Xgwm397-4A Xgwm397 Xgwm397a Xgwm397b gwm397
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGTCATGGATTATTTGGTCGG 3' 5' CTGCACTCTCGGTATACCAGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS397
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring