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GrainGenes Marker Report: Xgwm400

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Locus	Xgwm400
Type	Microsatellite
Chromosome	7B
Chromosome Arm	7BL
Map	Svevo/Ciccio-7B 0 [ Show all 6 ]
	[ Show Nearby Loci ]
Breakpoint Interval	C-7BL7-0.63
Map Data	Durum wheat, Svevo x Ciccio [ Show all 6 ]
Probe	WMS400
Possible Orthologs	Xgwm400-7B gwm400 gwm400-7B
Probe for Locus	WMS400
Locus	Xgwm400 Xgwm400-7B gwm400 gwm400-7B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTGCTGCCACCACTTGC 3' 5' TGTAGGCACTGCTTGGGAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS400
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring