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GrainGenes Marker Report: Xgwm415

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Locus	Xgwm415
Type	Microsatellite
Chromosome	5A
Map	Ta-NxW-5A 70.1 Wheat, UC1110 x PI610750 5A 41.5 Wheat-Composite2004-5A 27
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMS415
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm415-5A Xgwm415-5B gwm415 gwm415-5A
Probe for Locus	WMS415
Locus	Xgwm415-5A Xgwm415 Xgwm415-5B gwm415 gwm415-5A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)25imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GATCTCCCATGTCCGCC 3' 5' CGACAGTCGTCACTTGCCTA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS415
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring