GrainGenes Marker Report: Xgwm425-2A
Locus
Xgwm425-2A
Type
Microsatellite
Chromosome
2A
Map
Chinese_Spring_Deletion_SSR_2A -0.39 Ta-SSR-2004-2A 51.9 Ta-Synthetic/Opata-BARC-2A 77.8 Ta-Synthetic/Opata-GPW-2A 108.5 Ta-Synthetic/Opata-SSR-2A 96.1
[ Show Nearby Loci ]
Breakpoint Interval
C-2AS5-0.78
Map Data
Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe
WMS425
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-2A, the intervals most likely to contain locus are Xbcd152 - Xgwm71.2 or Xgwm71.2 - Xbcd543
Possible Orthologs
Xgwm425 Xgwm425-2A.1 Xgwm425-2A.2 gwm425 gwm425-2A
Probe for Locus
WMS425
Locus
Xgwm425-2A
[ Show all 6 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GAGCCCACAAGCTGGCA 3'
5' TCGTTCTCCCAAGGCTTG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS425
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm425-2A
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