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GrainGenes Marker Report: Xgwm427

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Locus	Xgwm427
Type	Microsatellite
Chromosome	6A
Chromosome Arm	6AL
Map	Tt-LxG-6A 140.3 Wheat-Composite2004-6A 137
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Map Data	T. turgidum, Langdon x G18-16 Wheat, Composite, 2004
Probe	WMS427
Possible Orthologs	Xgwm427-6A gwm427 gwm427-6A
Probe for Locus	WMS427
Locus	Xgwm427 Xgwm427-6A gwm427 gwm427-6A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)31(CA)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AAACTTAGAACTGTAATTTCAGA 3' 5' AGTGTGTTCATTTGACAGTT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS427
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring