GrainGenes Marker Report: Xgwm427-6A
Locus
Xgwm427-6A
Type
Microsatellite
Chromosome
6A
Chromosome Arm
6AL
Map
Chinese_Spring_Deletion_SSR_6A 0.95
[ Show all 11 ]
[ Show Nearby Loci ]
Breakpoint Interval
6AL8-0.90-1.00
Map Data
Durum wheat, Kofa x UC1113
[ Show all 11 ]
Probe
WMS427
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-6A, the interval most likely to contain locus is between Xfba20 and Xcdo836
Possible Orthologs
Xgwm427 gwm427 gwm427-6A
Probe for Locus
WMS427
Locus
Xgwm427 Xgwm427-6A gwm427 gwm427-6A
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)31(CA)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AAACTTAGAACTGTAATTTCAGA 3'
5' AGTGTGTTCATTTGACAGTT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS427
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm427-6A
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