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GrainGenes Marker Report: Xgwm43-7B

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Locus	Xgwm43-7B
Type	Microsatellite
Chromosome	7B
Chromosome Arm	7BL
Map	Chinese_Spring_Deletion_SSR_7B -0.135 [ Show all 6 ]
	[ Show Nearby Loci ]
Breakpoint Interval	C-7BS1-0.27
Map Data	Wheat, Consensus SSR, 2004 [ Show all 6 ]
Probe	WMS43
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm43 Xgwm43-4A gwm43 gwm43-7B
Probe for Locus	WMS43
Locus	Xgwm43-7B Xgwm43 Xgwm43-4A gwm43 gwm43-7B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks	Insert contains repeat (CA)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (CA)x22. Product size 180 bp in Chinese Spring.
Type	PCR SSR
PCR primers	5' CACCGACGGTTTCCCTAGAGT 3' 5' GGTGAGTGCAAATGTCATGTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS43
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring