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GrainGenes Marker Report: Xgwm455

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Locus	Xgwm455
Type	Microsatellite
Chromosome	2D
Map	Ta-NxW-2D 29.2 Wheat, UC1110 x PI610750 2D 7.5 Wheat-Composite2004-2D 26
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMS455
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm455-2D Xgwm455-6B gwm455 gwm455-2D
Probe for Locus	WMS455
Locus	Xgwm455-2D Xgwm455-6B Xgwm455 gwm455 gwm455-2D
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)19imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATTCGGTTCGCTAGCTACCA 3' 5' ACGGAGAGCAACCTGCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS455
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring