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GrainGenes Marker Report: Xgwm456-3D

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Locus	Xgwm456-3D
Type	Microsatellite
Chromosome	3D
Chromosome Arm	3DL
Map	CS/SQ1-3D 68 [ Show all 7 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QGpc.ccsu-3D.1
Map Data	Wheat, Chinese Spring x SQ1 [ Show all 7 ]
Probe	WMS456
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Salina E et al. (2000) Microsatellite mapping of the induced sphaerococcoid mutation genes in Triticum aestivum Theoretical and Applied Genetics 100:686-689. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-3D, the intervals most likely to contain locus are XksuA6 - Xmwg688 or Xmwg688 - Xbcd134
Possible Orthologs	Xgwm456 Xgwm456-1B
Probe for Locus	WMS456
Locus	Xgwm456-3D Xgwm456-1B Xgwm456 S-D1
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCTGAACATTACACAACCCTGA 3' 5' TGCTCTCTCTGAACCTGAAGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS456
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring