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GrainGenes Marker Report: Xgwm458

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Locus	Xgwm458
Type	Microsatellite
Map	Ta-NxW-1D 55 Wheat, Avalon x Cadenza 1D 57.1 Wheat, Finch x Eltan 1D.1 11.6
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Map Data	Wheat, Avalon x Cadenza Wheat, Finch x Eltan Wheat, Nanda2419 x Wangshuibai
Probe	WMS458
Possible Orthologs	Xgwm458-1D gwm458-1D gwm458
Probe for Locus	WMS458
Locus	Xgwm458-1D Xgwm458 gwm458-1D gwm458
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)13 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AATGGCAATTGGAAGACATAGC 3' 5' TTCGCAATGTTGATTTGGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS458
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring