GrainGenes Marker Report: Xgwm473-7A
Locus
Xgwm473-7A
Type
Microsatellite
Map
T.b.xT.m.-7A 153.8
[ Show Nearby Loci ]
Map Data
T. boeoticum x monococcum
Probe
WMS473
Possible Orthologs
Xgwm473
[ Show all 6 ]
Probe for Locus
WMS473
Locus
Xgwm473-2A
[ Show all 7 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)14(TTGG)(GT)8 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' TCATACGGGTATGGTTGGAC 3'
5' CACCCCCTTGTTGGTCAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS473
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm473-7A
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