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GrainGenes Marker Report: Xgwm480

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Locus	Xgwm480
Type	Microsatellite
Chromosome	3A
Map	Wheat-Composite2004-3A 105
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS480
Possible Orthologs	Xgwm480-3A gwm480 gwm480-3A
Probe for Locus	WMS480
Locus	Xgwm480-3A Xgwm480 gwm480 gwm480-3A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)16(CA)13 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGCTGCTACTTGTACAGAGGAC 3' 5' CCGAATTGTCCGCCATAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS480
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring