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GrainGenes Marker Report: Xgwm484

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Locus	Xgwm484
Type	Microsatellite
Chromosome	2D
Map	Ta-NxW-2D 94.6 Wheat-2008-Coda-Brundage-2D 0 Wheat-2008-Corrigin-CS(T2D)-2D 57 Wheat-Composite2004-2D 31 Wheat_BranchedSpike_2009_2D 19.8
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Map Data	Wheat 2008, Coda x Brundage, C gene Wheat 2008, Corrigin x CS(T2D), C gene Wheat, Branched spike gene, 2009 Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS484
Possible Orthologs	Xgwm484-2D gwm484
Probe for Locus	WMS484
Locus	Xgwm484-2D Xgwm484 gwm484
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)29 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACATCGCTCTTCACAAACCC 3' 5' AGTTCCGGTCATGGCTAGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS484
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring