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GrainGenes Marker Report: Xgwm493-3B

Locus
Xgwm493-3B
Type
Microsatellite
Chromosome
3B
3B Triticum aestivum
Chromosome Arm
3BS
Map
Chinese_Spring_Deletion_SSR_3B-0.89
[ Show all 17 ]
[ Show Nearby Loci ]
QTL with Locus Marker
QFhs.ndsu-3B
QFhs.ndsu-3BS
QFhs.ndsu.3B
Breakpoint Interval
3BS-8/3BS3
3BS8-0.78-1.00
Map Data
Durum wheat, Kofa x UC1113
[ Show all 18 ]
Species
Triticum aestivum
Probe
WMC493
WMS493
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
[ Show all 6 ]
Data Source
Roeder, Marion S.98.09.15
Remark
It is not clear why this locus has two probes. Curator note. May 2018
Possible Orthologs
Xgwm493
Xgwm493-3B.1
Xgwm493-3B.2
gwm493
gwm493-3B



Probe for Locus
WMC493
Locus
Xgwm493-3B
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25g9
Donor: Serasem
Trimmed to insert at both ends. Bases 4 to 97 (complement) show homology to 3c4 bases 65 to 158 (94 in longest run, maxScore = 77). (TC)5 104 to 113, (CA)19 139 to 176, (CT)11 117 to 138.
Type
PCR
SSR
PCR primers
Forward AcATAcAATgggATTccAgcAg at 57 length 22 bases, Tm 63
Reverse AAcAAAcggTgTTcATgcAAgT at 221 length 22 bases, Tm 63
SSR size
186 bases expected, Chinese Spring
Amplification Conditions
61C anneal, smear (120-200 bases)
Sequence
WMC493
Source Species
Triticum aestivum