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GrainGenes Marker Report: Xgwm494-4A

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Locus
Xgwm494-4A
Type
Microsatellite
Chromosome
4A
Map
Conan/Reeder-4A86.6169
T.b.xT.m.-4A99.5
Ta-Courtot/CS-GPW-4A-64.3
Ta-SSR-2004-4A49.1
[ Show Nearby Loci ]
Map Data
T. boeoticum x monococcum
Wheat, Conan x Reeder
Wheat, Consensus SSR, 2004
Wheat, Courtot x Chinese Spring, GPW
Probe
WMS494
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xgwm494
[ Show all 14 ]



Probe for Locus
WMS494
Locus
Xgwm494-4A
[ Show all 15 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)13
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' ATTGAACAGGAAGACATCAGGG 3'
5' TTCCTGGAGCTGTCTGGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS494
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring