GrainGenes Marker Report: Xgwm495.2-4B
Locus
Xgwm495.2-4B
Type
Microsatellite
Map
Conan/Reeder-4B 47.7267
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder
Probe
WMS495
Possible Orthologs
Xgwm495
[ Show all 6 ]
Probe for Locus
WMS495
Locus
Xgwm495.1-4B
[ Show all 7 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GAGAGCCTCGCGAAATATAGG 3'
5' TGCTTCTGGTGTTCCTTCG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS495
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm495.2-4B
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