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GrainGenes Marker Report: Xgwm497.2-1A

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Locus
Xgwm497.2-1A
Type
Microsatellite
Map
Conan/Reeder-1A189.367
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder
Probe
WMS497
Possible Orthologs
Xgwm497
[ Show all 22 ]



Probe for Locus
WMS497
Locus
Xgwm497.2-1A
[ Show all 23 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)29imp
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GTAGTGAAGACAAGGGCATT 3'
5' CCGAAAGTTGGGTGATATAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS497
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring