GrainGenes Marker Report: Xgwm499-5B
Locus
Xgwm499-5B
Type
Microsatellite
Chromosome
5B
Chromosome Arm
5BL
Map
Chinese_Spring_Deletion_SSR_5B 0.65
[ Show all 11 ]
[ Show Nearby Loci ]
Breakpoint Interval
5BL1-0.55-0.75
Map Data
Durum wheat, Kofa x UC1113
[ Show all 12 ]
Probe
WMS499
Reference
Data Source
Roeder, Marion S. 98.09.15
Possible Orthologs
Xgwm499
[ Show all 6 ]
Probe for Locus
WMS499
Locus
Xgwm499-5B
[ Show all 7 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)32 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' ACTTGTATGCTCCATTGATTGG 3'
5' GGGGAGTGGAAACTGCATAA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS499
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm499-5B
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