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GrainGenes Marker Report: Xgwm5

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Locus	Xgwm5
Type	Microsatellite
Chromosome	3A
Map	Wheat-Composite2004-3A 72
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS5
Possible Orthologs	Xgwm5-3A [ Show all 16 ]
Probe for Locus	WMS5
Locus	Xgwm5-3A [ Show all 16 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks	Insert contains repeat (TC)23(T)4(GT)12(GA)10 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (TC)x23(T)x4(GT)x12(GA)x10. Product size 172 bp in Chinese Spring.
Type	PCR SSR
PCR primers	5' GCCAGCTACCTCGATACAACTC 3' 5' AGAAAGGGCCAGGCTAGTAGT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS5
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring