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GrainGenes Marker Report: Xgwm508

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Locus	Xgwm508
Type	Microsatellite
Chromosome	6B
Map	Svevo/Ciccio-6B 50.5 Wheat-Composite2004-6B 98
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Breakpoint Interval	6BS5-0.76-1.05
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004
Probe	WMS508
Possible Orthologs	Xgwm508-6B gwm508 gwm508-6B
Probe for Locus	WMS508
Locus	Xgwm508-6B Xgwm508 gwm508 gwm508-6B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)19imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTTATAGTAGCATATAATGGCC 3' 5' GTGCTGCCATGATATTT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS508
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring