GrainGenes Marker Report: Xgwm515-2D
Locus
Xgwm515-2D
Type
Microsatellite
Chromosome
2D
Map
Chinese_Spring_Deletion_SSR_2D -0.735
[ Show all 6 ]
[ Show Nearby Loci ]
Breakpoint Interval
2DS5-0.47-1.00
Map Data
Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe
WMS515
Reference
Data Source
Roeder, Marion S. 98.09.15
Possible Orthologs
Xgwm515
[ Show all 7 ]
Probe for Locus
WMS515
Locus
Xgwm515-2A
[ Show all 8 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)17(TCAT)(GT)6 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AACACAATGGCAAATGCAGA 3'
5' CCTTCCTAGTAAGTGTGCCTCA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS515
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm515-2D
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