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GrainGenes Marker Report: Xgwm52

Locus	Xgwm52
Type	Microsatellite
Chromosome	3D
Map	Ta-NxW-3D 75.5 Wheat, Avalon x Cadenza 3D 0 Wheat-Composite2004-3D 64
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Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS52
Possible Orthologs	Xgwm52-3D
Probe for Locus	WMS52
Locus	Xgwm52-3D Xgwm52
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)4AT(GT)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CTATGAGGCGGAGGTTGAAG 3' 5' TGCGGTGCTCTTCCATTT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS52
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring