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GrainGenes Marker Report: Xgwm52-3D

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Locus
Xgwm52-3D
Type
Microsatellite
Chromosome
3D
Chromosome Arm
3DL
Map
Chinese_Spring_Deletion_SSR_3D0.54
[ Show all 9 ]
[ Show Nearby Loci ]
Breakpoint Interval
3DL2-0.27-0.81
Map Data
Wheat, Chinese Spring x SQ1
[ Show all 9 ]
Probe
WMS52
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source
Roeder, Marion S.98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-3D, the interval most likely to contain locus is between Xbcd134 and Xabc176
Possible Orthologs
Xgwm52



Probe for Locus
WMS52
Locus
Xgwm52-3D
Xgwm52
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)4AT(GT)20
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' CTATGAGGCGGAGGTTGAAG 3'
5' TGCGGTGCTCTTCCATTT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS52
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring