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GrainGenes Marker Report: Xgwm533.1

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Locus	Xgwm533.1
Type	Microsatellite
Map	Ta-NxW-3B 12 Wheat, UC1110 x PI610750 3B 5.8
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMS533
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	gwm533 [ Show all 11 ]
Probe for Locus	WMS533
Locus	Xgwm533-3B [ Show all 14 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)18(CA)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AAGGCGAATCAAACGGAATA 3' 5' GTTGCTTTAGGGGAAAAGCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS533
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring