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GrainGenes Marker Report: Xgwm547

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Locus	Xgwm547
Type	Microsatellite
Chromosome	3B
Map	Wheat, Avalon x Cadenza 3B 224.6 Wheat-Composite2004-3B 100 Wheat_YrH922_2019_exp 21.5
	[ Show Nearby Loci ]
Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat_YrH922_2019
Probe	WMS547
Possible Orthologs	gwm547 Xgwm547-2B Xgwm547-3B gwm547-3B
Probe for Locus	WMS547
Locus	Xgwm547-3B Xgwm547-2B Xgwm547 gwm547 gwm547-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)12 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTTGTCCCTATGAGAAGGAACG 3' 5' TTCTGCTGCTGTTTTCATTTAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS547
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring