GrainGenes Marker Report: Xgwm547-2B
Locus
Xgwm547-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BS
Map
Tturgidum-SSR-2B -80
[ Show Nearby Loci ]
Map Data
T. turgidum, Messapia x dicoccoides, SSR
Probe
WMS547
Possible Orthologs
gwm547 Xgwm547 Xgwm547-3B gwm547-3B
Probe for Locus
WMS547
Locus
Xgwm547-3B Xgwm547-2B Xgwm547 gwm547 gwm547-3B
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)12 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GTTGTCCCTATGAGAAGGAACG 3'
5' TTCTGCTGCTGTTTTCATTTAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS547
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm547-2B
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