GrainGenes Marker Report: Xgwm547-3B
Locus
Xgwm547-3B
Type
Microsatellite
Synonym
Synonym gwm547 gwm547-3B Xgwm547
Chromosome
3B
Map
Chinese_Spring_Deletion_SSR_3B 0.815
[ Show all 9 ]
[ Show Nearby Loci ]
QTL with Locus Marker
Qsst.msub-3BL
Breakpoint Interval
3BL7-0.63-1.00
Map Data
Durum wheat, Kofa x UC1113
[ Show all 8 ]
Probe
WMS547
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-3B, the intervals most likely to contain locus are Xfba235 - Xtam63 or Xtam63 - Xmwg11
Possible Orthologs
gwm547 Xgwm547 Xgwm547-2B gwm547-3B
Probe for Locus
WMS547
Locus
Xgwm547-3B Xgwm547-2B Xgwm547 gwm547 gwm547-3B
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)12 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GTTGTCCCTATGAGAAGGAACG 3'
5' TTCTGCTGCTGTTTTCATTTAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS547
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm547-3B
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