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GrainGenes Marker Report: Xgwm55

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Locus	Xgwm55
Type	Microsatellite
Chromosome	2B
Chromosome Arm	2BL
Map	Svevo/Ciccio-2B 93.8 Tt-LxG-2B 79.6 Wheat-Composite2004-2B 56
	[ Show Nearby Loci ]
Breakpoint Interval	C-2BS1-0.53
Map Data	Durum wheat, Svevo x Ciccio T. turgidum, Langdon x G18-16 Wheat, Composite, 2004
Probe	WMS55
Possible Orthologs	Xgwm55-1D [ Show all 21 ]
Probe for Locus	WMS55
Locus	Xgwm55-2B [ Show all 21 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (TC)3(T)3(CT)17 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GCATCTGGTACACTAGCTGCC 3' 5' TCATGGATGCATCACATCCT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS55
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring