GrainGenes Marker Report: Xgwm554-5B
Locus
Xgwm554-5B
Type
Microsatellite
Chromosome
5B
Chromosome Arm
5BL
Map
Chinese_Spring_Deletion_SSR_5B 0.775
[ Show all 7 ]
[ Show Nearby Loci ]
Breakpoint Interval
5BL9-0.76-0.79
Map Data
T. turgidum, Messapia x dicoccoides, SSR
[ Show all 7 ]
Probe
WMS554
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-5B, the interval most likely to contain locus is between Xmwg914 and Xbcd307
Possible Orthologs
Xgwm554
[ Show all 8 ]
Probe for Locus
WMS554
Locus
Xgwm554-5B
[ Show all 9 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)13(GT)14 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' TGCCCACAACGGAACTTG 3'
5' GCAACCACCAAGCACAAAGT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS554
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm554-5B
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