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GrainGenes Marker Report: Xgwm577

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Locus	Xgwm577
Type	Microsatellite
Chromosome	7B
Map	Svevo/Ciccio-7B 155.9 Ta-NxW-7B 159.8 Wheat, Avalon x Cadenza 7B 60.4 Wheat, WH542 x Synthetic 7B 60 Wheat-Composite2004-7B 105
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Breakpoint Interval	7BL10-0.78-1.00
Map Data	Durum wheat, Svevo x Ciccio Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, WH542 x Synthetic
Probe	WMS577
Possible Orthologs	Xgwm577-7B [ Show all 9 ]
Probe for Locus	WMS577
Locus	Xgwm577-7B [ Show all 12 ]
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)14(TA)6 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. In 369 Avena sterilis accessions there were 2 alleles of size 50-55 bp, PIC = negative.
Type	Genomic PCR SSR
PCR primers	5' ATGGCATAATTTGGTGAAATTG 3' 5' TGTTTCAAGCCCAACTTCTATT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS577
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring