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GrainGenes Marker Report: Xgwm583

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Locus	Xgwm583
Type	Microsatellite
Chromosome	5D
Map	Ta-NxW-5D 92 Wheat-Composite2004-5D 45
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS583
Possible Orthologs	Xgwm583-5D gwm583
Probe for Locus	WMS583
Locus	Xgwm583-5D Xgwm583 gwm583
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)27 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TTCACACCCAACCAATAGCA 3' 5' TCTAGGCAGACACATGCCTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS583
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring