GrainGenes Marker Report: Xgwm6-4B
Locus
Xgwm6-4B
Type
Microsatellite
Chromosome
4B
Chromosome Arm
4BL
Map
Chinese_Spring_Deletion_SSR_4B 0.93
[ Show all 12 ]
[ Show Nearby Loci ]
Breakpoint Interval
4BL1-0.86-1.00
Map Data
T. turgidum, JK x Cham1
[ Show all 12 ]
Species
Triticum aestivum
Probe
WMS6
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
[ Show all 6 ]
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-4B, the interval most likely to contain locus is distal to Xfba177
Possible Orthologs
WMS6
[ Show all 17 ]
Probe for Locus
WMS6
Locus
Xgwm6.1-4B
[ Show all 18 ]
Reference
General Remarks
Insert contains repeat (GA)40 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (GA)x40. Product size 205 bp in Cheyenne. In 369 Avena sterilis accessions there were 3 alleles of size 48-60 bp, PIC = negative.
Type
Genomic PCR SSR
PCR primers
5' CGTATCACCTCCTAGCTAAACTAG 3'
5' AGCCTTATCATGACCCTACCTT 3' CGTATCACCTCCTAGCTAAACTAG
AGCCTTATCATGACCCTACCTT
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS6
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm6-4B
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