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GrainGenes Marker Report: Xgwm604

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Locus	Xgwm604
Type	Microsatellite
Chromosome	5B
Map	Wheat-Composite2004-5B 107
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS604
Possible Orthologs	Xgwm604-1B Xgwm604-5B gwm604 gwm604-5B
Probe for Locus	WMS604
Locus	Xgwm604-5B Xgwm604 Xgwm604-1B gwm604 gwm604-5B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)29 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TATATAGTTCAATATGACCCG 3' 5' ATCTTTTGAACCAAATGTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS604
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring