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GrainGenes Marker Report: Xgwm614-2A

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Locus	Xgwm614-2A
Type	Microsatellite
Chromosome	2A
Chromosome Arm	2AS
Map	Chinese_Spring_Deletion_SSR_2A -0.89 [ Show all 7 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QPro.inra-2A
Breakpoint Interval	2AS5-0.78-1.00
Map Data	Durum wheat, Svevo x Ciccio [ Show all 8 ]
Species	Triticum aestivum
Probe	WMS614
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm614 [ Show all 14 ]
Probe for Locus	WMS614
Locus	Xgwm614 [ Show all 16 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)23imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GATCACATGCATGCGTCATG 3' 5' TTTTACCGTTCCGGCCTT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS614
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring