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GrainGenes Marker Report: Xgwm614-2B

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Locus
Xgwm614-2B
Type
Microsatellite
Chromosome
2B
Map
Louise/Penawawa-2B134
Ta-SSR-2004-2B11.6
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Wheat, Louise x Penawawa
Probe
WMS614
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xgwm614
[ Show all 14 ]



Probe for Locus
WMS614
Locus
Xgwm614
[ Show all 16 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)23imp
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GATCACATGCATGCGTCATG 3'
5' TTTTACCGTTCCGGCCTT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS614
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring