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GrainGenes Marker Report: Xgwm63

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Locus	Xgwm63
Type	Microsatellite
Map	Svevo/Ciccio-7A 141.2
	[ Show Nearby Loci ]
Breakpoint Interval	7AL21-0.74-0.86
Map Data	Durum wheat, Svevo x Ciccio T. boeoticum x monococcum
Probe	WMS63
Possible Orthologs	Xgwm63-7A gwm63
Probe for Locus	WMS63
Locus	Xgwm63-7A Xgwm63 gwm63
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)17(TA)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCGACCTGATCGCCCCTA 3' 5' CGCCCTGGGTGATGAATAGT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS63
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring