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GrainGenes Marker Report: Xgwm636

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Locus
Xgwm636
Type
Microsatellite
Chromosome
2A
Map
Svevo/Ciccio-2A8.4
Wheat-Composite2004-2A18
Wheat_Yr69_2016-2A0
[ Show Nearby Loci ]
Breakpoint Interval
C-2AS5-0.78
Map Data
Durum wheat, Svevo x Ciccio
Wheat, Composite, 2004
Wheat_Yr69_2016
Probe
WMS636
Possible Orthologs
Xgwm636-2A
gwm636
gwm636-2A



Probe for Locus
WMS636
Locus
Xgwm636-2A
Xgwm636
gwm636
gwm636-2A
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)28imp
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' CGGTAGTTTTTAGCAAAGAG 3'
5' CCTTACAGTTCTTGGCAGAA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS636
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring