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GrainGenes Marker Report: Xgwm642

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Locus	Xgwm642
Type	Microsatellite
Chromosome	1D
Map	Ta-NxW-1D 73.1 Wheat-Composite2004-1D 82
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
2 Point Data	Dn9/Xgwm642
Probe	WMS642
Possible Orthologs	Xgwm642-1D gwm642-1D gwm642
Probe for Locus	WMS642
Locus	Xgwm642-1D Xgwm642 gwm642-1D gwm642
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)14 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACGGCGAGAAGGTGCTC 3' 5' CATGAAAGGCAAGTTCGTCA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS642
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring