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GrainGenes Marker Report: Xgwm644b

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Locus
Xgwm644b
Type
Microsatellite
Chromosome
6B
Map
Wheat-Composite2004-6B127
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS644
Possible Orthologs
gwm644
[ Show all 11 ]



Probe for Locus
WMS644
Locus
Xgwm644-6B
[ Show all 12 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)20
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GTGGGTCAAGGCCAAGG 3'
5' AGGAGTAGCGTGAGGGGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS644
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring