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GrainGenes Marker Report: Xgwm645

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Locus	Xgwm645
Type	Microsatellite
Chromosome	3D
Map	Wheat, Avalon x Cadenza 3D 7.7 Wheat, UC1110 x PI610750 3D 68.9 Wheat-Composite2004-3D 81
	[ Show Nearby Loci ]
Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMS645
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm645-3D gwm645
Probe for Locus	WMS645
Locus	Xgwm645-3D Xgwm645 gwm645
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)23imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGACCGGAAAAGGGCAGA 3' 5' GCCCCTGCAGGAGTTTAAGT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS645
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring