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GrainGenes Marker Report: Xgwm666b-3A

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Locus
Xgwm666b-3A
Type
Microsatellite
Chromosome
3A
Chromosome Arm
3AS
Map
Tdicoccoides-FHB-QTL-3AS125.1
[ Show Nearby Loci ]
Map Data
T.dicoccoides, FHB QTL
Probe
WMS666
Reference
ReferenceOtto CD et al. (2002) Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat Plant Molecular Biology 48:625-632.
Possible Orthologs
Xgwm666
[ Show all 17 ]



Probe for Locus
WMS666
Locus
Xgwm666-3A
[ Show all 18 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)13
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GCACCCACATCTTCGACC 3'
5' TGCTGCTGGTCTCTGTGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS666
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring