GrainGenes Marker Report: Xgwm67-5B
Locus
Xgwm67-5B
Type
Microsatellite
Chromosome
5B
Map
Chinese_Spring_Deletion_SSR_5B 0.145 Ta-SSR-2004-5B 65.2 Ta-Synthetic/Opata-BARC-5B 33.6 Ta-Synthetic/Opata-GPW-5B 30.9 Ta-Synthetic/Opata-SSR-5B 39.45
[ Show Nearby Loci ]
Breakpoint Interval
C-5BL6-0.29
Map Data
Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe
WMS67
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-5B, the interval most likely to contain locus is between Xgwm335 and Xabc164
Possible Orthologs
Xgwm67 Xgwm67-3A gwm67 gwm67-5B
Probe for Locus
WMS67
Locus
Xgwm67-5B Xgwm67 Xgwm67-3A gwm67 gwm67-5B
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)10 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' ACCACACAAACAAGGTAAGCG 3'
5' CAACCCTCTTAATTTTGTTGGG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS67
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm67-5B
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