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GrainGenes Marker Report: Xgwm72-3B

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Locus
Xgwm72-3B
Type
Microsatellite
Chromosome
3B
Map
Chinese_Spring_Deletion_SSR_3B-0.165
[ Show all 6 ]
[ Show Nearby Loci ]
Breakpoint Interval
C-3BS1-0.33
Map Data
Wheat, Consensus SSR, 2004
[ Show all 6 ]
Probe
WMS72
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source
Roeder, Marion S.98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-3B, the intervals most likely to contain locus are Xcdo328 - Xpsr903 or Xpsr903 - XATPase.2
Possible Orthologs
gwm72
Xgwm72



Probe for Locus
WMS72
Locus
Xgwm72-3B
Xgwm72
gwm72
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)48imp
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' TGGTCCCTCTCCCTTTCTCT 3'
5' ACAGAATTGAAGATTGTCGGTC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS72
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring