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GrainGenes Marker Report: Xgwm77

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Locus	Xgwm77
Type	Microsatellite
Chromosome	3B
Chromosome Arm	3BS
Map	Ta-NxW-3B 118.8 Tt-LxG-3B 69.2 Wheat-Composite2004-3B 46
	[ Show Nearby Loci ]
Map Data	T. turgidum, Langdon x G18-16 Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS77
Possible Orthologs	gwm77 Xgwm77-3B gwm77-3B
Probe for Locus	WMS77
Locus	Xgwm77 Xgwm77-3B gwm77 gwm77-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)10(GA)40imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACAAAGGTAAGCAGCACCTG 3' 5' ACCCTCTTGCCCGTGTTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS77
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring