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GrainGenes Marker Report: Xgwm77-3B

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Locus	Xgwm77-3B
Type	Microsatellite
Chromosome	3B
Chromosome Arm	3BS
Map	Chinese_Spring_Deletion_SSR_3B -0.165 [ Show all 7 ]
	[ Show Nearby Loci ]
Breakpoint Interval	C-3BS1-0.33
Map Data	Wheat, Arina x Forno [ Show all 7 ]
Species	Triticum aestivum
Probe	WMS77
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-3B, the intervals most likely to contain locus are Xcdo328 - Xpsr903 or Xpsr903 - XATPase.2
Possible Orthologs	gwm77 Xgwm77 gwm77-3B
Probe for Locus	WMS77
Locus	Xgwm77 Xgwm77-3B gwm77 gwm77-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)10(GA)40imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACAAAGGTAAGCAGCACCTG 3' 5' ACCCTCTTGCCCGTGTTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS77
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring