GrainGenes Marker Report: Xgwm99.2
Locus
Xgwm99.2
Type
Microsatellite
Chromosome
5D
Map
Wheat-Composite2004-5D 90
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS99
Possible Orthologs
Xgwm99 Xgwm99-1A Xgwm99.1 gwm99 gwm99-1A
Probe for Locus
WMS99
Locus
Xgwm99-1A
[ Show all 6 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AAGATGGACGTATGCATCACA 3'
5' GCCATATTTGATGACGCATA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS99
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm99.2
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