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GrainGenes Marker Report: Xmwg502

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Locus	Xmwg502
Type	RFLP
Chromosome	5R
Chromosome Arm	5RS
Map	Hordeum-IxT/Q-5H -46.4 Rye-2009-Consensus-5R 48.7 Secale-PxP-update-5R 3
	[ Show Nearby Loci ]
Map Data	Barley, IxT/QTL Rye, P87 x P105, update Rye_2009_consensus
Probe	MWG502
Possible Orthologs	MWG502 Xmwg502-5R
Probe for Locus	MWG502
Locus	MWG502 Xmwg502 Xmwg502-5R
Reference	Marcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500. Sayed-Tabatabaei BE et al. (1998) DNA sequencing and primer designing for RFLP clones evenly distributed in the barley genome. Barley Genetics Newsletter 28:15-18. Graner A et al. (1991) Construction of an RFLP map of barley Theoretical and Applied Genetics 83:250-256.
General Remarks	Restriction Enzyme for CAPS marker is Msp I; Hpa II
Type	Genomic STS
PCR primers	5' tgtttagctaagctgttgctgagg 3' 5' ctctgaagcatggaaccagatttg 3'
STS primers	T7 side (5' to 3') ATCAAGATGAGGAAGCTGATGCAGT T3 side (5' to 3') TCTTGTCTGAAGCATGGAACCAGAT
Amplification Conditions	5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 55 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence	AJ234471 AJ234470 MWG502 MWG502-5 MWG502-3
Linkage Group	5H
Insert Enzyme	PstI
Source Species	Hordeum vulgare
Insert Size	0.9